Track 16:Other Screening Techniques
Other Screening Techniques: The following are some other Approaches to The Experimental Pharmacology.
Avian chick embryo model: Utlization of chick models for Genetic Manipulation is continued. The further studies include, Time Lapse Video Microscopy, knockout studies, TALENs, CRISPRs, Zinc-Finger nuclease technology
Stem cell models: Lund Human Mesencephalic (LUHMES) cell lines, a 3D dopaminergic neuronal model for neurotoxicity testing and Parkinson's disease (PD). Using confocal microscopy, gene expression, and flow cytometry, the 3D model was characterized and observed a highly reproducible differentiation process, which begins the further era of studying some of the diseases.
In chemico tests:
The toxic potential of some substances can sometimes be detected using relatively simple chemistry based methods and not requiring human cells or the whole cell lines. A major effort in this project is to measure in chemico reactivity. The HPLC and Direct Peptide Reactivity Assay (DPRA) are some of the well established techniques.
1. Pyrogen tests
2. Monocyte-Activation Test
3. Limulus Amebocyte Lysate (LAL) test
4. Carcinogenicity Testing
5. Cell transformation Assays